Molecular self-assembly with DNA is an attractive route for building nanodevices with user-defined functionalities. DNA-based objects featuring complex three-dimensional (3D) nanoscale shapes in which thousands of DNA basepairs (bp) are placed with subnanometer-precision at designed positions have been successfully fabricated. A key challenge for a DNA-based approach to nanotechnology is the production of desired objects at the gram scale at affordable prices to enable applications in areas ranging from chemistry to health. To meet this challenge here we propose a scientific engineering approach that combines biophysical assembly studies with process engineering to elucidate basic assembly principles and process limitations. The production of single-stranded DNA (ssDNA) molecules plays a key role for creating DNA-based nanodevices. The objectives of the engineering partner (DWB) include the selection and optimization of ssDNA production principles, the development of ssDNA production processes for large-scale preparation of ssDNA, and the reaction- engineering characterization of several production approaches to yield quantitative data for scale-up and economic evaluations. The objectives of the science partner (HD) comprise exploring the influence of DNA strand composition, sequences, and lengths on assembly processes, and determining reaction schemes that lead to time / reactant efficient production of high-quality objects. The project will focus on the scaled-up production of objects for use in other projects within the Focus Area Biomaterials.